Figure 5

Clustering of Oryza species and OryzaTPS1s. Hierarchical cluster analysis was performed for the sesquiterpene products of seven OryzaTPS1s and the patterns of sesquiterpenes actively emitted from the seven Oryza species from which the corresponding OryzaTPS1s were cloned. Each enzyme clustered closely to its corresponding species showing that the products formed by in vitro enzyme assays were also produced by the intact plant. The name of each unique enzyme and its corresponding rice species were depicted with the same color. Relative abundance of each sesquiterpene was expressed as a percentage of the total amount of the four products. The color range represented percentages from 0% (a target compound was not detected at the expected retention time) to 100% abundance. Average values from three replicates were used.