Figure 6

In vitro PsDFR and PsLAR coupled assays. Product synthesis rates from the coupled assays were measured using DFR substrates, dihydroquercetin (A) and dihydromyricetin (B). Left: pseudo-kinetics plots were inferred from the coupled assays. Each data point represents means ± SE (n = 3). C-F: LC-MS [M + H]⁺ extracted ion chromatographs (C and D m/z = 291; E and F, m/z = 307) of authentic (+)-catechin (D) and (+)-gallocatechin (F) along with in vitro assay products (C and E) from PsDFR only (red line) or PsDFR + PsLAR coupled assays (blue line).