Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhiziinfection

Table 5 Primer set designed to gene isolation and transgene detection

Target	Orientation	Tm (°C)	Primer Sequence	PCR product size (pb)
35S	Forward	52	5'-GGACCCCCACCCACGAGGAG-3'	139*
GmWRKY27 overexpression	Forward	58	5'- CACCATGGATTATTCATCATGGATTAACA-3'	921
GmWRKY27 overexpression	Reverse		5'- TTAATTATTATTGTGCAACATTTTTC-3'
WRKY (RNAi)	Forward	58	5'- CACCCTTCCTTGGATCTCAACATTAATCT -3'	176
WRKY (RNAi)	Reverse		5'- TAACTTCTTGTTTTCTGCACTCACC -3'
Intron	Forward	60	5'- TGCCTCTTCTTACGGCTTTCTGTG -3'	400
Intron	Reverse		5' - TGCCGTCTGTGATGGCTTCCA -3'
Hpt	Forward	60	5'-GCGATTGCTGATCCCCATGTGTGTAT-3'	512
Hpt	Reverse		5'-GGTTTCCACTATCGGCGAGTACTT-3'

*Fragment length was considered from the beginning of the primer sequence until the end of the promoter sequence.

ISSN: 1471-2229