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Figure 2 | BMC Plant Biology

Figure 2

From: Functional characterisation of Arabidopsis SPL7 conserved protein domains suggests novel regulatory mechanisms in the Cu deficiency response

Figure 2

Biochemical analysis of SPL7 subcellular localization and processing. (a) Total protein extracts from tobacco leaves transiently expressing different GFP-tagged SPL7 versions were subjected to biochemical fractionation, as described in Experimental Procedures and analysed by Western blotting with antibodies against GFP (α-GFP). Antibodies against the organelle markers TPR7 (α-TPR7), PEPC (α-PEPC) and histone H3 (α-H3) were used to validate the fractionation. M, microsomes; C, cytosol; N, nucleus. Main bands are highlighted with an asterisk. (b) Proteolytic processing of transiently expressed GFP::SPL7 and SPL7::GFP by Western blotting. WT, protein extract of non-transformed tobacco leaves. Sizes of molecular-weight markers run in the same gels are shown alongside the blots in (a-b) according to manufacturer's indications for 10% SDS-PAGE gels.

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BMC Plant Biology

ISSN: 1471-2229

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