Amplicon position in the sex locus and its boundaries on chromosome 2 of the 12×.0 reference sequence version. a) VVIB23 SSR marker (light blue rectangle) and amplicon position (red ellipses); b) Amplicon position and gene Gaze annotation in the 143 kb sex locus defined by Fechter et al. . The 12×.0 annotated genes version are represented in dark blue and our amplicons in red. For the APT3 and the ETO1gene, we used the synteny between the chromosome 2 of the 8X reference sequence version, the unassembled scaffold_233 of the 12×.0 reference sequence version, and the BAC sequencing maps of V. riparia and V. cinerea to estimate their relative position on chromosome 2, 12×.0 version (see Methods). The phosphatidic phosphatase 2 (PAP2), is not predicted by the Gaze annotation of the 12×.0 reference sequence version but it is annotated by the Gaze annotation of the 8× reference sequence version and confirmed by Fechter et al.  on the 12×.0 version.