Purification of recombinant ClpT1. Expression and purification of the recombinant protein ClpT1 were evaluated by gel electrophoresis and Coomassie staining. Soluble extracts from uninduced and induced cultures were loaded in lanes 2 and 3, respectively. Lane 4 shows the eluted protein after affinity chromatography and lane 5, ClpT1 after the whole purification procedure. Molecular weight markers were loaded on lane 1; their molecular weights are stated on the left.