Figure 6
From: Isolation and functional characterization of a high affinity urea transporter from roots of Zea mays
Growth of ZmDUR3 mod -expressed in the dur3-3 Arabidopsis mutant. The Arabidopsis dur3 mutant, atdur3-3 [18], was transformed with ZmDUR3 mod -ORF under the control of the CaMV 35S-promoter. (a) Growth of the wild type Col-0 (WT), atdur3-3 mutant line and three ZmDUR3 mod -overexpressing lines (atdur3-3 + ZmDUR3-A, -B, -C) on sterile half strength MS medium supplied with 1 μM NiCl2 and 50 μM NO3- and different concentrations of urea or 0.5 mM ammonium nitrate (AN) as a sole N source. (b) Effect of urea treatment on root morphology in Arabidopsis plants grown with 0.5 mM urea. Plants were grown for 16 days on nutrient agar-medium.