Phylogenetic tree of DUR3 urea transporters. A phylogentic analyses was performed using the DUR3 amino acid sequences of Saccharomyces cerevisiae (Sc, AAA34582), Zea mays (Zm, KJ652242), Oryza sativa (Os, NP_001065513), Arabidopsis thaliana (At, NP_199351) and putative DUR3 orthologs from Aegilops tauschii (Aegt, EMT22254), Triticum urartu (Tu, EMS63712.1), Hordeum vulgare (Hv, BAJ94433.1), Brachypodium distachyon (Bd, XP_003571687), Setaria italica (Si, XP_004965066), Sorghum bicolor (Sb, XP_002438118), Cucumis sativus (Cs, XP_004146194.1), Vitis vinifera (Vv, XP_002263043), Populus trichocarpa (Pt, XP 002303472.1), Solanum lycopersicum (Sl, XP_004245999), Prunus persica (Pp, EMJ11521.1), Medicago truncatula (Mt, XP_003612583), Glycine max (Gm, XP_003523904). The tree was constructed by aligning the protein sequences by Clustal-W and the evolutionary history was inferred using the Neighbor-Joining method. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Poisson correction method and are in the units of the number of amino acid substitutions per site.