Molecular and phenotypic analysis of SA transgenic cassava coupled expression of cytosolic Me Cu/ZnSOD and cytosolic Me APX2 genes. (a) Schematic presentation of the T-DNA region of pC-P54::MeCu/ZnSOD-35S::MeAPX2 with unique XbaI site. LB, left border; RB, right border of T-DNA; T35S, CaMV 35S terminator; TNOS, NOS terminator ; P35S, cauliflower mosaic virus 35S promoter; P54/1.0, vascular-specific promoter p54/1.0; HPT, hygromycin phosphotransferase. (b) Southern blot analysis of transgenic and WT cassava plants for transgene integration. Transgene integration patterns in SA lines detected XbaI-digested genomic DNA by HPT (left panel) and MeAPX2 (right panel) probes. gAPX, genomic DNA of cassava ascorbate peroxidase; T-APX2, transgene MeAPX2; M, λ HindIII DIG-labeled molecular marker; P, plasmid pC-P54::MeCu/ZnSOD-35S::MeAPX2; WT, wild-type control. Numbers indicate different transgenic lines. (c) Plant growth status and phenotype evaluation in field. WT, wild-type control; SA1, SA2 and SA4, independent transgenic cassava plant lines.