Genic distribution of 24-nt siRNAs. Relative densities of 24-nt siRNAs in 5′ 1-kb upstream regions, gene body regions and 3′ 1-kb downstream regions were calculated separately on sense (right lane) and antisense (left lane) strands for 0-, 3-, 5-DAP kernels and 7-, 10-, 15-DAP endosperms in protein-coding genes, pseudo-genes and TEs, respectively. The results indicate 24 nt-siRNAs were highly enriched in the 1-kb upstream and downstream regions in antisense strands, and the density of 24-nt siRNAs in the 10-DAP endosperm were much higher than those in other two stages of endosperm. Orange-red: 5′ 1-kb upstream regions, Orange: gene body regions, bluish gray: 3′ 1-kb downstream regions. Red line: 0-DAP kernel, brown line: 3-DAP kernel, green line: 5-DAP kernel, blue line: 7-DAP endosperm, dark blue: 10-DAP endosperm, dark red: 15-DAP endosperm.