Figure 8

RNA levels of miR159 , MYB33 and MYB101 in wrky2 mutants and wild type. RNA was extracted from seedlings on MS medium without ABA or with 1.5 μM ABA 4 d or 7 d post-stratification. (A) Each lane contained 20 μg total RNA. Each experiment also was executed three times. (B) Relative RNA levels of WRKY2 were analyzed using gene-specific primers by real-time PCR. Three independent experiments are shown by reextracting RNA from other samples. Each experiment also was executed three times.