Immuno-localization of WP5212 in developing wheat grain (cv AC Barrie) and peanut cotyledon (cv Valencia). (A) Wheat aleurone (al) cells were positively stained while endosperm (es) remained unstained when sections were stained with WP5212 antibodies. (B) Wheat aleurone layer and endosperm treated with preimmune serum (PIS). Green staining in seed coat (sc) shows unspecific staining. (C) Wheat embryonic (em) tissue stained with WP5212 antibodies. (D) Wheat embryonic cells treated with PIS (E) Peanut cotyledon (cot) stained with WP5212 antibodies. (F) Peanut cotyledon treated with PIS. Green (examples indicated with arrowheads) indicates positive staining.