A set of EST-SNPs for map saturation and cultivar identification in melon

BMC Plant Biology

Table 1 Amplicons designed from ESTs for SNP discovery

	Amplicons	Failed	Monomorphic	Polymorphic	Polymorphic amplicons*	In silico SNP validation
Random ESTs	223	58	72	93	56.3%
in silico pSNPs	269	41	69	159	69.7%	51.8%
in silico pSCHs	97	14	57	26	31.3%	21.3%
TOTAL	589	113	198	278	58.4%

ESTs were selected at random or chosen because they contained pSNPs or pSCHs in the MELOGEN database. Columns show the number of amplicons that failed to amplify or gave bad quality sequences, and monomorphic and polymorphic amplicons between SC and PS. The percentages of polymorphic amplicons and in silico SNPs that were validated are shown in the last two columns. (*) Polymorphic amplicons rate was calculated without considering failed amplicons.

ISSN: 1471-2229