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Table 1 Comparison of miRNA expression using MiRCURY array and TaqMan miRNA assay

From: Profiling microRNA expression in Arabidopsis pollen using microRNA array and real-time PCR

  MiRCURY Array£ Taqman MiRNA Assay¥    
  log2(sample/pool) MP CT Inf CT   AS Slotkin et. al
Name Inf 1 Inf 2 MP1 MP2 mean mean ΔΔCT RP§ Inf MP
156a-f -0.44 0.27 0.23 0.19 29.85 29.77 -0.9 2 2591 51961
156g -0.33 0.34 0.16 0.09 35.08 34 0.09* 0 131 678
156h      35.49 31.6 2.89 0 1525 611
157a-d -0.32 0.92 -1.35 -1.04 34.42 31.18 2.26 1 1792 671
158a 0.07 0.77 -2.21 -2.12 32.87 27.9 3.95 8 16305 2307
158b 0.15 0.79 -1.82 -1.82 32.89 30.5 1.39 0 13757 2037
159a -0.33 1.03 -3.17 -3.04 NA 25.2   67 197957 5540
159b -0.31 1.04 -3.31 -3.01 NA 24.3   10 189644 5319
159c -0.17 1.07 -2.87 -2.84 35.85 29.7 4.49 0 35850 1247
160a-c -0.04 0.39 -0.31 -0.39 30.71 28.95 0.74 17 833 351
161 -0.03 1.04 -1.29 -1.36 33.65 29.7 2.91 71 8245 4737
162a,b 0.05 0.73 -1.13 -1.2 35.35 28.9 5.82 7 123 35
163 0.39 0.51 -3.27 -2.83 36.98 30.5 5.29 3 5665 126
164a,b NA NA NA NA 36.39 25.6 8.53 21 10771 382
164c NA NA NA NA 37.04 26.1 10.03 1 10005 349
165a,b 0.31 0.58 0 0.06     1 1 15
166a-g -0.24 1.11 -3.04 -3.25 31.93 24.9 6.4 6 472 33
167a,b -0.64 0.96 -2.4 -2.29 35.95 25.6 9.48 218 33681 1154
167c -0.34 0.94 -1.41 -1.55 NA 26.1   1 250 14
167d -0.09 0.85 -0.76 -0.96 NA 34   4 852 19
168a,b 0.37 0.36 -0.7 -1.18 32.49 28.6 3.56 16 19462 5038
169a 0.07 0.83 -0.91 -0.94 NA 33.8   1 399 7
169b,c NA NA NA NA NA 32.5   0 380 7
169d-g -0.01 0.52 -0.74 -0.98 NA 30.01   2 234 9
170 NA NA NA NA 32.9 29.3 2.95 7 5207 137
171a 0 0.79 -2.7 -2.67 37.0 26.5 9.81 247 39570 922
171b,c 0.14 0.51 -1.6 -1.52 36.28 26.8 8.40 5 4149 91
172a,b 0.08 0.79 -1.47 -1.55 32.69 28.9 3.14 73 8220 8147
172c,d NA 0.26 -0.36 -0.33 32.47 28.4 3.47 1 7576 8353
172e 0.04 0.75 -1.42 -1.48 32.81 28.9 3.17 6 5245 2320
173 -0.38 0.39 -1.99 -1.93 34.93 29.6 4.71 1 625 501
319a,b 0.07 0.33 -0.93 -0.97 36.18 27.44 8.26 5 14514 713
319c      36.97 30.11 6.38 2 13795 653
390a,b 0.14 0.66 -1.39 -1.33 33.15 24.86 7.79 2 8743 695
391 0.15 0.42 -0.6 -0.54     3 14 19
394a,b NA 0.42 -0.93 -0.96 35.84 28.97 6.43 1 21 1
395a,d,e -0.04 0.11 -0.49 -0.47 NA 34.91   0 139 2
395b,c,f -0.09 0.22 0.01 -0.07 NA 31.94   0 137 2
396a NA 0.72 NA -0.76 34.87 29.34 4.91 6 352 19
396b 0.06 1.18 -0.92 -1.39 NA 27.5   4 371 21
399b,c -0.21 0.09 -0.31 NA 35.84 32.12 2.91 0 485 106
403 NA NA NA NA 36.61 30.9 4.92 1 696 275
414 0.18 0.11 -0.42 -0.66     NA 0 0
419 0.17 0.31 -0.47 -0.32 NA NA   NA 0 0
447a,b -0.13 0.11 -0.04 -0.15     0 286 49
447c -0.26 -0.1 0.22 0.23 NA NA   0 0 0
  1. All miRNA names have been abbreviated. If a probe represents a miRNA family with more than three members, the name is shortened to save space. For example, miR156a-f represents miR156a, b, c, d, e, f. MiRNAs that were not present either on the array or the Taqman assay were in bold. £, the MiRCURY array data were shown as Log2(sample/pool). ¥, The CT value represents means from all the replicates. T-test was performed to evaluate the ΔΔCT value for difference that is statically significant (with p < 0.05). The only one that did not pass the test was marked by an asterisk. §, the value in the table represents the normalized read (reads/million) for each miRNA in inflorescence (Col-0) as stored in ASRP database. , the value represents the un-normalized sequencing reads from the small RNA libraries published by Slotkin et. al. [29]. The whole inflorescence library has 4,158,848 sequences, while the mature pollen library has 1,034,665 sequences. The table only listed miRNAs that were found in at least one of the sources. Inf, inflorescence; MP, mature pollen. NA, the miRNA was either not detected or unavailable.