Characterization and isolation of a T-DNA tagged banana promoter active during in vitro culture and low temperature stress

Table 1 Luciferase (LUC) activation during three developmental stages in 10 promoter tagged lines of 'Three Hand Planty'

Based on the direct correlation between the number of greyscale levels as detected by the CCD camera and the level of LUC activity, lines were ranked at 26°C in five different classes: Not detectable (N), weak (W), moderate (M), strong (S) and very strong (VS). At each developmental stage real-time monitoring of LUC activity took place while subjecting to LT at 8°C (see Figure 1B) and 2 h later LUC activity was scored again. Upward and downward-pointing arrows indicate an increase and decrease, respectively, relative to the LUC activity at 26°C.
LUC activity detected according to upper greyscale limit setting: not detectable (N) – less than 500, weak (W) – between 500 and 3000, moderate (M) – between 3000 and 5000, strong (S) – between 5000 and 10,000, very strong (VS) – more than 10,000.
^aCell colony stage on ZZ medium 3 months after transformation.
^bDifferentiation stage on RD2 medium 8 months after transformation.
^cIn vitro regenerated plantlet on REG medium 15 months after transformation.
^dLine transformed with positive control vector pETKUL3 [luc⁺ gene fused to the enhanced Cauliflower Mosaic Virus (CaMV) 35S RNA promoter].

ISSN: 1471-2229