Based on the direct correlation between the number of greyscale levels as detected by the CCD camera and the level of LUC activity, lines were ranked at 26°C in five different classes: Not detectable (N), weak (W), moderate (M), strong (S) and very strong (VS). At each developmental stage real-time monitoring of LUC activity took place while subjecting to LT at 8°C (see Figure 1B) and 2 h later LUC activity was scored again. Upward and downward-pointing arrows indicate an increase and decrease, respectively, relative to the LUC activity at 26°C.
LUC activity detected according to upper greyscale limit setting: not detectable (N) – less than 500, weak (W) – between 500 and 3000, moderate (M) – between 3000 and 5000, strong (S) – between 5000 and 10,000, very strong (VS) – more than 10,000.
aCell colony stage on ZZ medium 3 months after transformation.
bDifferentiation stage on RD2 medium 8 months after transformation.
cIn vitro regenerated plantlet on REG medium 15 months after transformation.
dLine transformed with positive control vector pETKUL3 [luc+ gene fused to the enhanced Cauliflower Mosaic Virus (CaMV) 35S RNA promoter].