Effective carbon partitioning driven by exotic phloem-specific regulatory elements fused to the Arabidopsis thaliana AtSUC2 sucrose-proton symporter gene

Table 2 Analysis of transient carbohydrates in control and complemented lines

Representative line	Carbohydrate	Lamina	petiole
WT	Glu	2.28 ± 0.95	0.51 ± 0.02
(Col-0)^a	Fru	0.80 ± 0.36	0.14 ± 0.03
	Suc	1.72 ± 0.76	0.35 ± 0.07
	Starch^b	59.53 ± 4.96	8.28 ± 3.68
	Total	64.33 ± 4.42	9.28 ± 3.79
SUC2p::cSUC2	Glu	1.40 ± 0.42	0.53 ± 0.15
(line 1039)^a	Fru	0.42 ± 0.12	0.12 ± 0.02
	Suc	1.40 ± 0.40	0.35 ± 0.07
	Starch	84.60 ± 18.20^c	12.02 ± 3.64
	Total	87.83 ± 18.17^c	13.02 ± 3.60
CoYMVp::cSUC2	Glu	1.11 ± 0.22	0.55 ± 0.08
(line 1070)	Fru	0.41 ± 0.13	0.14 ± 0.03
	Suc	1.90 ± 0.07	0.43 ± 0.04
	Starch	46.55 ± 4.50^d	10.92 ± 2.55
	Total	49.96 ± 4.20^d	12.04 ± 2.56
RolCp::cSUC2	Glu	15.41 ± 4.24^c	0.67 ± 0.15
(line 1133)	Fru	3.44 ± 1.05^c	0.17 ± 0.04
	Suc	11.50 ± 1.79^c	0.52 ± 0.03^c
	Starch	547.63 ± 117.90^c	34.50 ± 6.80^c
	Total	577.97 ± 113.92^c	35.87 ± 6.98^c

Sugar and starch in the lamina (excluding midrib) and petiole of the indicated plant lines after 30 days of growth. The first three mature leaves from each plant were pooled for extraction, and variation (standard deviation) calculated from n = 3 to 5 sibling plants. Sugars levels are expressed as nmoles per milligram fresh weight, and starch is expressed as glucose equivalents.
^a [25]
^b Expressed as glucose equivalents
^c Significant increase relative to wild type; Students T-test, p < 0.05
^d Significant decrease relative to wild type; Students T-test, p < 0.05

ISSN: 1471-2229