Figure 3

Northern analyses of a few select forward subtracted SSH cDNA clones. RNA isolated from the leaves of the control and 425 mM NaCl-treated plants and blotted onto Hybond N⁺ membrane was hybridized with the individual radiolabelled ESTs. A RNA blot each for the control and NaCl-treated sample was hybridized with PCR amplified radiolabelled actin fragment. The horizontal bars against the individual genes represent increase (in%) in transcripts of the respective genes in response to NaCl treatment of the plant when compared to control. The values were obtained after normalization of the blot intensities of actin for the control and NaCl treated sample.