Figure 2
Acyltransferase activity from transformants expressing either LPEAT1, LPEAT2 or control empty vector. The data represent triplicate samples with products separated on TLC plates and quantified by Instant Imager autoradiography. The mean values are presented to the left of each horizontal bar with standard error in parenthesis and in italics. The concentration of 16:0-CoA and 18:1-CoA were 22.7 μM in all assays except for the assay with 16:0-LPA as an acceptor in the yeast slc1 strain, which were 2.25 nmol of [14C]18:1-CoA (75000 dpm). The amount of microsomal protein added to the assays of the ale1 transformants were: for LPS, 1.88 μg, LPC and LPE, 1.33 μg, and LPA, 1.16 μg, and in the assay of LPA as an acceptor in the slc1 transformants, LPEAT1, LPEAT2 and empty vector control, 2, 2.08, 1.5 μg respectively.