Immunoblot analysis of Chinese Spring deletion lines of the short arm of chromosome 1 and 6. (A) Using mAb Glia-α9. (B) Using mAb Glia-α20. CS: Chinese Spring wild type. Arrowheads indicate absent protein bands. (C) Physical maps of the short (S) arms of wheat chromosomes 1A, 1B, 1D, 6A, 6B, and 6D from centromer to telomeric ends (Wheat Genetic and Genomic Resources Centre, Kansas State University, USA). Arrows on the right of each chromosome indicate the deletion lines with their breakpoint (indicated as fraction length from the centromer). The banding patterns within the chromosomes are according to Gill et al.