Table 3 Characteristics of cultivated peanut (Arachis hypogaea L.) EST-SSR and efficiency of markers development
From: Utility of EST-derived SSR in cultivated peanut (Arachis hypogaea L.) and Arachiswild species
Motif | No. of EST-SSRs | No. of designed primers | No. amplified EST-SSRs (%) | No. polymorphic EST-SSRs (%) | ||
|---|---|---|---|---|---|---|
| Â | Â | Â | Cultivated peanut | Wild species | Cultivated peanut | Wild species |
Di | 288 | 55 | 42 | 42 | 10 | 34 |
AC/GT | 14 | 6 | 5 | 5 | 2 | 4 |
AG/CT | 218 | 39 | 29 | 29 | 8 | 24 |
AT/AT | 56 | 10 | 8 | 8 | 0 | 6 |
Tri | 563 | 221 | 196 | 196 | 14 | 174 |
AAC/GTT | 37 | 14 | 11 | 11 | 0 | 9 |
AAG/CTT | 153 | 59 | 51 | 51 | 2 | 43 |
AAT/ATT | 105 | 27 | 24 | 24 | 4 | 23 |
ACC/GGT | 68 | 32 | 29 | 29 | 2 | 28 |
ACG/CTG | 17 | 4 | 3 | 3 | 0 | 3 |
ACT/ATG | 64 | 26 | 24 | 24 | 2 | 21 |
AGC/CGT | 29 | 10 | 9 | 9 | 3 | 9 |
AGG/CCT | 29 | 16 | 15 | 15 | 0 | 11 |
AGT/ATC | 43 | 23 | 21 | 21 | 1 | 19 |
CCG/CGG | 18 | 10 | 9 | 9 | 0 | 8 |
Tetra | 15 | 5 | 5 | 5 | 1 | 5 |
AAAG/CTTT | 7 | 1 | 1 | 1 | 0 | 1 |
AAAT/ATTT | 2 | 2 | 2 | 2 | 1 | 2 |
AATC/AGTT | 3 | 1 | 1 | 1 | 0 | 1 |
AATT/AATT | 1 | 0 | 0 | 0 | 0 | 0 |
ACAT/ATGT | 2 | 1 | 1 | 1 | 0 | 1 |
Penta-type | 6 | 3 | 3 | 3 | 0 | 3 |
AAAAG/CTTTT | 1 | 1 | 1 | 1 | 0 | 1 |
AAAAT/ATTTT | 2 | 0 | 0 | 0 | 0 | 0 |
AGTAT/ATATC | 3 | 2 | 2 | 2 | 0 | 2 |
Hexa-type | 9 | 6 | 5 | 5 | 1 | 5 |
AAAAAG/CTTTTT | 1 | 1 | 1 | 1 | 0 | 1 |
AAGACG/CTGCTT | 2 | 1 | 1 | 1 | 1 | 1 |
AATAGT/ATCATT | 2 | 2 | 1 | 1 | 0 | 1 |
AATGAT/ACTATT | 3 | 1 | 1 | 1 | 0 | 1 |
AGCAGT/ATCGTC | 1 | 0 | 0 | 0 | 0 | 0 |
AGCTCC/AGGTCG | 1 | 1 | 1 | 1 | 0 | 1 |
Total | 881 | 290 | 251 | 251 | 26 | 221 |