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Table 3 Characteristics of cultivated peanut (Arachis hypogaea L.) EST-SSR and efficiency of markers development

From: Utility of EST-derived SSR in cultivated peanut (Arachis hypogaea L.) and Arachiswild species

Motif No. of EST-SSRs No. of designed primers No. amplified EST-SSRs (%) No. polymorphic EST-SSRs (%)
    Cultivated peanut Wild species Cultivated peanut Wild species
Di 288 55 42 42 10 34
AC/GT 14 6 5 5 2 4
AG/CT 218 39 29 29 8 24
AT/AT 56 10 8 8 0 6
Tri 563 221 196 196 14 174
AAC/GTT 37 14 11 11 0 9
AAG/CTT 153 59 51 51 2 43
AAT/ATT 105 27 24 24 4 23
ACC/GGT 68 32 29 29 2 28
ACG/CTG 17 4 3 3 0 3
ACT/ATG 64 26 24 24 2 21
AGC/CGT 29 10 9 9 3 9
AGG/CCT 29 16 15 15 0 11
AGT/ATC 43 23 21 21 1 19
CCG/CGG 18 10 9 9 0 8
Tetra 15 5 5 5 1 5
AAAG/CTTT 7 1 1 1 0 1
AAAT/ATTT 2 2 2 2 1 2
AATC/AGTT 3 1 1 1 0 1
AATT/AATT 1 0 0 0 0 0
ACAT/ATGT 2 1 1 1 0 1
Penta-type 6 3 3 3 0 3
AAAAG/CTTTT 1 1 1 1 0 1
AAAAT/ATTTT 2 0 0 0 0 0
AGTAT/ATATC 3 2 2 2 0 2
Hexa-type 9 6 5 5 1 5
AAAAAG/CTTTTT 1 1 1 1 0 1
AAGACG/CTGCTT 2 1 1 1 1 1
AATAGT/ATCATT 2 2 1 1 0 1
AATGAT/ACTATT 3 1 1 1 0 1
AGCAGT/ATCGTC 1 0 0 0 0 0
AGCTCC/AGGTCG 1 1 1 1 0 1
Total 881 290 251 251 26 221