Figure 6
Aniline blue staining of alamethicin-treated BY-2 cells. (A, C) shows cells pretreated with DMSO as solvent control while in (E, G) cells have been pre-treated with oryzalin. A callose synthase reaction was performed before staining, but in C and G, EGTA was added before the start of the reaction, to chelate the Ca2+ present. (B), (D), (F) and (H) are close ups for (A), (C), (E) and (G) respectively. Bars in (G) and (H) are size markers for the respective columns.