Figure 4
From: FORCA, a promoter element that responds to crosstalk between defense and light signaling
Infections with virulent Hyaloperonospora parasitica suppress FORCA-mediated transcriptional activity. A-D: GUS staining (A&B) of plants treated with H20 (A) or HpNoco2 (B). Trypan blue staining (C&D) of plants treated with H20 (C) or HpNoco2 (D). E: Fluorometric analysis of GUS specific activity in protein extracts of 3xFORCA-pCAMBIA plants subjected to mock-treatment or sprayed with HpNoco2 (2*104 spores/ml). Mean and standard error were calculated from four pooled data points generated in two independent experiments, each with two transgenic lines. Based on T-tests the differences were significant (p = 0.0036).