Figure 3

Effects of virus infections on P-miR164a activity. L56 and L35 P-miR164a::GUSArabidopsis transgenic lines and 35S::GUS control transgenic plants were virus-inoculated to quantify the effects in P-miR164a activity. (A) The bar chart shows the GUS activity mean value and standard error (SE) obtained in each group with n ≥ 10 from at least two biological replicates. Values were normalized to mock-inoculated controls of each line. Statistical comparisons were made by Kruskal-Wallis test with Dunn's post-test. Statistical differences between treated and mock-treated groups are shown. **p < 0.05, ***p < 0.01 compared to mock controls. (B) Representative RT-PCR of the pre-miR164a transcript in L56 transgenic plants. The housekeeping EF1α gene was amplified as an internal control. (C) Quantitative RT-PCR analysis to measure the level of the pre-miR164a in Arabidopsis thaliana Col 0 plants after ORMV infection. The chart shows the normalized CTs ± SE for each condition and the expression ratio between them calculated with REST algorithm.