Table 1 Description of A. thaliana Ler mutant lines and mutations in relation to EMS dose.
EMS dose (mM) | Number of TILLer lines | Mean (B+C) fertility class (%) | Total number of screened lines* | Number of mutations | Het/Hom mutation ratio | Number of lines with two mutations | Density of mutations per line |
|---|---|---|---|---|---|---|---|
20 | 46 | 24.5 ± 5.4 | 614 (1.5%) | 7 (1.5%) | 6.0 | 0 (0%) | 1/84 kb |
25 | 2082 | 16.8 ± 3.7 | 21150 (50.8%) | 178 (39.6%) | 3.6 | 10 (29.4%) | 1/114 kb |
30 | 741 | 10.5 ± 2.8 | 9196 (22.1%) | 112 (24.9%) | 3.3 | 10 (29.4%) | 1/79 kb |
35 | 739 | 3.7 ± 1.2 | 9313 (22.4%) | 128 (28.4%) | 4.3 | 12 (35.3%) | 1/70 kb |
40 | 104 | 2.6 ± 1.0 | 1335 (3.2%) | 25 (5.6%) | 4.0 | 2 (5.9%) | 1/51 kb |
Total | 3712 | 12.6 ± 4.6** | 41608 | 450 | 3.7** | 34 | 1/96 kb** |