Cytosolic N-terminal arginine-based signals together with a luminal signal target a type II membrane protein to the plant ER

Table 1 Comparison of the cytosolic tail sequence and transmembrane domain length of glucosidases I cloned from different species

Organism	Cytosolic tail sequence	TMD length
Arabidopsis thaliana AJ278990	MTGASRRSARGRIKSSSLSPGSDEGSAYPPSIRRGKGKELVSIGAFKTNLK	18
Oryza sativa BAB86175.1	MSGGGGSSSVRRPVAAARSRSGPEPDARRAAAAAAAAAAAAARRRGRGDHGPLRLMEVSPRN	23
Neurospora crassa CAC18158.1	MAPPPPRQPRQ	23
Strongylocentrotus Purpuratus XP_797552.1	>MAARTRIADSGGGARSRETKTKPKSGNGAQSRNNETQSSSKN	23
Danio rerio XP_696318.1	MGRRRKRVATGDGVPSPRKEEKAPAPPRKEKKKKTDIGK	24
Apis melifera XP_623340.1	MSILNISITVLCIAIATWFSYKGYLETRVNTPYDIKKLVTIS	23
Tribolium castaneum XP_972740.1	MARQRRTQGAADPNKGTNSSSSNGSNSTNNRSSKSTS	23
Enchytraeus japonensis BAE93517.1	MAKKKVPREKNHSGGTTRRTSESSSNNHADSKRQIRIKLNEKRKRQEPGSK	23
Caenorhabditis elegans NP_502053.1	MHREHEEMHQPSRRRRPPREVERPSATIRYEPVAEPEPWCSFCSWD	23
Homo sapiens NP_006293.2	MARGERRRRAVPAEGVRTAERAARGGPGRRDGRGGGPR	21
	-60 -50 -40 -30 -20 -10 -1

Numbers below the cytosolic tail sequences indicate the position from the transmembrane domain (TMD). Bold letters highlight the importance of arginine (R) and lysine (K) residues. Note underlined sequence from H. sapiens retains a reporter membrane protein in the ER in plant cell.

ISSN: 1471-2229