Figure 4

Amplicon melting analysis of fragment ABD6-1. Amplicon melting analysis of fragment ABD6-1 in duplicated non-mutant and mutant samples, showing the normalized melting curve (A), difference plot (B) and derivative melting curve (C). Non-mutants are shown in red and black (thick lines). Mutants are (as in Table 2) No3 C83T (blue), No4 G103A (green), No5 C133T (salmon, one PCR did not work, only one sample shown), No6 G147A (brown), No7 G147A (magenta), No8 G166A (purple), No9 C169T (aqua) and No10 C177T (orange).