An alignment of three homoeologous sequences of SSII. A gene fragment of SSII from primer ABDF6 to ABDR9 is aligned to show three homoeologous loci with 17 SNPs (gray highlighting). The codons (CAG and TGG) which can mutate to premature stop codons are indicated in boxes. Arrows indicate the positions of primers designed for PCR and HRM analysis. The four primer pairs are: ABDF6 and ABDR1, ABDF12 and ABDR22, ABDF2 and ABDR9, and ABDF3 and ABDR9.