Figure 4

Analysis of transcript abundance of flowering-time genes in transgenic (TG) and control Arabidopsis plants in long day conditions 8 or 16 h after DEX application. a) CO b) FT c) SOC1. Relative transcript accumulation is shown 8 h after DEX was sprayed onto 15 day- old plants growing in hydroponic media. Plants were treated with DEX (+DEX) or control solutions (-DEX) at ZT8 and harvested at ZT16. d) FT transcript accumulation is shown either 8 h (ZT16) or 16 h (ZT24) after DEX was sprayed onto 18 day-old plants growing in hydroponic media in LD. Plants were treated with DEX (+DEX) or control solutions (-DEX) at ZT8. Transcript abundance was quantified using qRT-PCR and expression levels were normalised to ACTIN2. The data is presented as mean +/- SD of 2 qRT-PCR runs for a and b) and a single run for c). The black bar on the harvest scheme indicates night, the open bar indicates day and the grey bar indicates the length of treatment with DEX or control solutions, ZT0 is lights on.