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Table 1 List of primers utilized in the expression analysis

From: Dominance induction of fruitlet shedding in Malus × domestica (L. Borkh): molecular changes associated with polar auxin transport

Gene Tm N°cycles Primer forward Primer reverse Accession number
MdLAX1 57 31 5'-AGTGCTATCAGTGCAAGC-3' 5'-CCGGAGAATTTTTCTGACTG-3' EF406263
MdLAX2 57 31 5'-CTACCACCACCACATCAC-3' 5'-GGAAACACCACCAATTCAC-3' EF406264
MdLAX3 57 31 5'-CATCACTAAGCTTCTCTCTC-3' 5'-CAACACCAATCCACTCTTC-3' EF406262
MdPIN1 71 33 5'-ATTTTACCACGGCGGGCAAGG-3' 5'-CCTTCCCGCCATTGTTGTCCT-3' EF406255/EF406256
MdPIN10 71 35 5'-CGGCGGACAGAATAATGCAGT-3' 5'-GAAGATCCTTCCCGCCATTGG-3' EF406260/EF406261
MdPIN4 71 37 5'-ACAATTCAACAGCAGCAGCA-3' 5'-CGGTCCCACCAAAAACATGG-3' EF406257
MdPIN7 71 32 5'-TTTGGGTTTTATCCCGCGCAGA-3' 5'-CGGCGGCTGCCTGATTTTTGC-3' EF406258/EF406259
MdIAA3 62 29 5'-GGTTGAGAATCATGAAGGGT-3' 5'-AAAGCCCGAGCTCTATGTCT-3' DQ848594
MdIAA7 64 30 5'-AATGCAAGAACAGGAGCTGA-3' 5'-TCCAATCAGTCGAACAACCT-3' DQ848598
MdAHS 58 30 5'-CTTCGTTGGGTCAATTACG-3' 5'-GGCAATAATCACTCCAAGG-3' EF406254
MdMAX2 61 30 5'-ACATTGCGGAAGCTCTTC-3' 5'-GCATCTTCGAATCGACAAC-3' DY242015
MdARR6 61 30 5'-GCATCATCATCTCCCTCCTCT-3' 5'-CAATCCATCGTGTGCAATCTC-3' EF406267
MdARR3/4 61 30 5'-TGATCCGACGATTGCTCCATC-3' 5'-CAGCGTTGGTCATTTTCAACC-3' EF406265/EF406266
MdUBI 61 28 5-CATCCCCCCAGACCAGCAGA-3 5-ACCACGGAGACGCAACACCAA-3 DQ438989
r18S 62 25 5-GTTACTTTTAGGACTCCGCC-3 5-TTCCTTTAAGTTTCAGCCTTG-3  
  1. Specific primers utilized for the expression analysis. Tm indicates the annealing temperature used in the PCR reaction. The number (N°) of cycles is that utilized in the experiments reported. The accession number is also reported in the right column.