Table 2 Information for nine markers that were sequenced from a set of 68 L. sativa accessions.
From: Association mapping and marker-assisted selection of the lettuce dieback resistance gene Tvr1
Marker | EST/Contig in CGPDB | Primers (5' - 3') | Ta (°C) | Mg (mM) | Amplicon size (bp) |
|---|---|---|---|---|---|
LK1457 | QG_CA_Contig4638 | F - AGGAGCAAAGGAAAGGCTTC | 57 | 1.5 | 636-648 |
R - TGCAACTTCTTCAGCCAATG | |||||
Cntg10044 | CLS_S3_Contig10044 | F - GCATGCCGATTACTCCTTTC R - TCCCCAATCACCTAAGATGG | 57 | 1.5 | 845-860 |
QGG19E03 | QGG19E03.yg.ab1 | F - ATATCCCACCGCCCATAGAT | 57 | 1.5 | 711-720 |
R - ACGCAACTAACCCGTTTCAT | |||||
Cntg4252 | CLS_S3_Contig4252 | F - GGGGAGTTCAGACGTTCAGT | 57 | 1.5 | 1160 |
R - CGAATTGATACACCGCAAAA | |||||
Cntg10192 | CLS_S3_Contig10192 | F - CTCGTTTTCAACACCGACAA | 57 | 1.5 | 349 |
R - TTGTCTCCGGCACTGTATCATCG | |||||
CLSM9959 | CLSM9959.b1_N18.ab1 | F - TGCTCAATTACACTCGAACCA | 57 | 1.5 | 326 |
R - CTTCATGGAGAGAAATACAAGGTC | |||||
CLSZ1525 | CLSZ1525.b1_J22.ab1 | F - TTGTTGAAATTATAAACACGAAGCA | 57 | 3 | 499-629 |
R - CAACAAAGGATGTCTCAAATTCA | |||||
QGC11N03 | QGC11N03.yg.ab1 | F - GCACCTGATGGCTGAATATG | 57 | 1.5 | 569-581 |
R - CATCCTCAATCGCTTGTGTT | |||||
Cntg11275 | CLS_S3_Contig11275 | F - GGAGAAATTTTGGAGCTGTAATTAC | 61 | 1.5 | 765-956 |
R - GGAGGTATGTTGAGGTACATGAC |