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Table 4 Comparison of the mutation frequencies obtained through the LI-COR and polyacrylamide/ethidium bromide screening method.

From: A modified TILLING approach to detect induced mutations in tetraploid and hexaploid wheat

   LI-COR Polyacrylamide/Ethidium bromide
Gene Region Sequence screened (kb) Mut. Mutation Frequency Sequence screened (kb) Mut. Mutation Frequency
WKS1 Kinase 930.0 18 1/52 kb 842.3 20 1/42 kb
  START 852.5 28 1/30 kb 390.1a 15 1/26 kb
WKS2 Kinase 998.4 25 1/40 kb 897.0 25 1/36 kb
  START 1053.7 25 1/42 kb 941.3 14 1/67 kb
Total/mean   3835 96 1/39.9 kb 3071 74 1/41.5 kb
  1. a 384 M2 plants were screened
  2. Four target regions where examined in the same 768 M2 plants from the hexaploid TILLING population and the total sequence screened was adjusted according to each method (see text).