Table 4 Comparison of the mutation frequencies obtained through the LI-COR and polyacrylamide/ethidium bromide screening method.
From: A modified TILLING approach to detect induced mutations in tetraploid and hexaploid wheat
| Â | Â | LI-COR | Polyacrylamide/Ethidium bromide | ||||
|---|---|---|---|---|---|---|---|
Gene | Region | Sequence screened (kb) | Mut. | Mutation Frequency | Sequence screened (kb) | Mut. | Mutation Frequency |
WKS1 | Kinase | 930.0 | 18 | 1/52 kb | 842.3 | 20 | 1/42 kb |
| Â | START | 852.5 | 28 | 1/30 kb | 390.1a | 15 | 1/26 kb |
WKS2 | Kinase | 998.4 | 25 | 1/40 kb | 897.0 | 25 | 1/36 kb |
| Â | START | 1053.7 | 25 | 1/42 kb | 941.3 | 14 | 1/67 kb |
Total/mean | Â | 3835 | 96 | 1/39.9 kb | 3071 | 74 | 1/41.5 kb |