Table 4 Gene and EST-based marker primer sequences. Primers used for DNA amplification in the markers obtained from Fragaria gene or EST or Prunus EST sequences.

Fragaria gene

EKO

ACAGTCCAGCTCCAATAGTTCC

GCTTTCCCATTGATTCTTGTCC

AY462247

F3H

GAGTTGATACCAAGCTCATCTCG

GTCACCTCTCTCCATCCTTCC

AB097151

DFR

CCACTCCTATGGATTTTGAGTCC

CTAGCACCCCATTTATTGTTGG

AB095030

ADH

GCKTCAMGAATTATYGGKGTTG

ATGGGASTTKRTGGGTGATG

X15588

Fragaria EST

EFvVB2179

ATCTGCGTGACAATGCAAAG

AAGAGCCTTCAGTTGCTCCA

CX662179

EFvVB2119

GCTCGAGCTGATTACGATTACC

TAAAGGACCCATCAGAGAAACG

CX662119

EFvNH8894

GGTTAGGTCTCCCCAGTGGT

GGACGTCTCCCACTAGCATC

DV438894

EFvVB1231

CCAACTGTGACATCCACGAC

GCTGTCACGCAGAAAATCAA

CX661231

EFaUF6868

GCTCTTCCAGGTCGAGTACG

GTTTCCACTTGGGCAGTTGT

CO816868

EFaTR1976

GTTGGTGCTGAGTTTGGTGA

CCCAACTGCTCAAGAAGGAG

CO381976

EFaUF7699

GTTCTTGTTGGTGGAAGCAC

CCTCAAAGACCTGAATGGAG

CO817699

EFaUF7084

CAGAAGAGGTTCAAGTTCC

ACACCATAGCAAGCCCTG

CO817084

EFvNH8484

TTCTGGTGTCGGCAAGTC

AGGCCTGCTCAACATTGG

DV438484

EFvVB2013

GTGCAGTTGCCAAAGGAGC

AGCTGGGTTTGCTGCTT

CX662013

EFvNH9852

TTCTGTCGTGGTGTCCC

ATGATCTTTTGGCGACCA

DV439852

EFvNH7822

GATGCTGGGTCTGCTGGG

GCCTGCTCATTGGCATA

CO817822

EFaUF7248

ACTGCTCGCCCAATGAAG

TCACATCAGCATCCATGTCA

CO817248

EFavB1923

GGCCGTGTCTCTTGCAGT

TGGGAGCAAATCCACCTT

CX661923

ACO

AGCACCTTCTACCTCAAACACC

CTCACAGAACAAGTCCAAGAGC

AF129073

Prunus EST

EPpCU9642

TTCAGTTGGCAGATCCTGTG

TGCTGAGACCCTTCCAATTT

BU039642

EPpCU1785

TTTTCCAAACCTTGCTGGAG

GCAGTAGCTGTGGCAATGAA

BU041785

EPpCU7308

GGCAGGCCGCTCTTATACTA

GACTCTTTTCGGGGTTCCA

BU047308

EPpCU9257

CACCACCGTTTCAAAAGAGG

CTGAAGCTCTAGCTGAGGCAAG

BU039257

EPpCU1830

TGATGCAATTGGCACAAAGC

CCTATCACCACTTACTTCACTGC

BU041830

EPpCU9910

ATAACTCTGCCATCCGAATCC

CATTCCTTGAACAGATCCTTGC

BU039910

EPpCU9223

AACAGAGCCAAGCTTATGCAG

TTTCTGCGCAACCGCATC

BU039223

EPpCU2875

AACTCAGAGACATATCTGCACAGG

AAGTTGAAGCGGTCTTCATAGG

BU042875