Figure 3

Expansion of the IR region in Polygonaceae. Ethidium bromide stained 1.5% agarose gel showing PCR amplification of rps15-ycf1 and ycf1-ndhF spacers for selected Polygonaceae taxa compared to Spinacia oleracea. 1, 2 – Fagopyrum esculentum ssp. ancestrale rps15-ycf1 and ycf1-ndhF fragments respectively, 3, 4 – F. homotropicum SSC rps15-ycf1 and ycf1-ndhF, 5, 6 – F. tataricum rps15-ycf1 and ycf1-ndhF, 7, 8 – Persicaria macrophylla rps15-ycf1 and ycf1-ndhF, 9, 10 – Rheum tanguticum rps15-ycf1 and ycf1-ndhF, 11, 12 – Reynoutria japonica rps15-ycf1 and ycf1-ndhF, 13, 14 – Coccoloba uvifera rps15-ycf1 and ycf1-ndhF, 15, 16 – Spinacia oleracea rps15-ycf1 and ycf1-ndhF (ycf1-ndhF – no amplification). M is the 0.25 – 10 Kb DNA ladder (SibEnzyme Ltd, Moscow, Russia), lowermost visible lane corresponds to 0.5 Kb.