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Table 6 Conservation and transferability of the new SSR markers across related taxa of coffee

From: Development of new genomic microsatellite markers from robusta coffee (Coffea canephoraPierre ex A. Froehner) showing broad cross-species transferability and utility in genetic studies

Species Coffea spp. Psilanthus spp.  
  Erythrocoffea Mozambicoffea Pachycoffea Melanocoffea   Paracoffea   
SSR C. arabica C. congensis Average C taxa C. eugenioides C. kapakata C. racemosa C. salavatrix Average C taxa C. excelsa C. liberica C. abeokuteae C. dewevrei C. arnoldiana C. aruwemiensis Average C taxa C. stenophylla Average C taxa ( Coffea) P. bengalensis P. wightiana Average C taxa ( Psilanthus) Average C taxa (for all coffees)
CaM02 + + 1.00 + + + + 1.00 + + + + - + 0.83 + 0.92 + + 1.00 0.93
CaM03 + + 1.00 - + + + 0.75 + + + + + + 1.00 + 0.92 - + 0.50 0.87
CaM08 + + 1.00 + + + + 1.00 + + + + - + 0.83 + 0.92 + - 0.50 0.87
CaM09 + + 1.00 + - + + 0.75 + + + + - + 0.83 + 0.85 - - 0.00 0.73
CaM11 + + 1.00 + + + + 1.00 + + + + + + 1.00 + 1.00 - - 0.00 0.87
CaM18 + + 1.00 + + + + 1.00 + + + + - + 0.83 - 0.85 + + 1.00 0.87
CaM20 + + 1.00 - - + - 0.25 - + + + - - 0.50 - 0.46 - - 0.00 0.4
CaM23 + + 1.00 + + + + 1.00 + + + + + - 0.83 + 0.92 + + 1.00 0.93
CaM25 + - 0.50 + + + + 1.00 + + + + + + 1.00 + 0.92 + + 1.00 0.93
CaM31 + + 1.00 + + + + 1.00 + + + + + + 1.00 + 1.00 - + 0.50 0.93
CaM33 + - 0.50 + + + + 1.00 + + + + + + 1.00 + 0.92 - + 0.50 0.87
CaM36 + + 1.00 + + + + 1.00 + + + + + - 0.83 + 0.92 + - 0.50 0.87
CaM42 + + 1.00 + + + + 1.00 - + + + + + 0.83 + 0.92 + + 1.00 0.93
CaM45 + + 1.00 - + + + 0.75 + - + + + + 0.83 + 0.85 + + 1.00 0.87
CaM49 + + 1.00 + + + + 1.00 + + + + + - 0.83 + 0.92 - - 0.00 0.8
CaM53 + - 0.50 - + + + 0.75 + - + + + - 0.67 + 0.69 + + 1.00 0.73
CaM54 - - 0.00 - + + - 0.50 - - - + - - 0.17 - 0.23 - + 0.50 0.27
CaM55 + + 1.00 + + + + 1.00 + + + + + + 0.83 - 0.85 + - 0.50 0.8
CaM57 + + 1.00 + + + + 1.00 - + + + - - 0.50 + 0.77 + + 1.00 0.8
CaM58 + + 1.00 + + + + 1.00 + + + + + + 1.00 + 1.00 - + 0.50 0.93
24 SSRs other than listed above + + 1.00 + + + + 1.00 + + + + + + 1.00 + 1.00 + + 1.00 1.00
Average T mark 0.98 0.91 0.94 0.89 0.95 1.00 0.95 0.95 0.91 0.93 0.98 1.00 0.84 0.84 0.91 0.91 0.93 0.80 0.84 0.82 0.92 (T mark-taxa )/0.91 (C taxa-mark )
  1. +/-: Indicates 'amplification'/'No amplification' and are given a weightage of 1 and 0 for transferability/conservence calculations respectively; T mark : Marker transferability over all the taxa; C taxa : Marker conservance over all the taxa; Tmark-taxa: Marker transferability of all the markers over all the taxa; Ctaxa-mark: Primer conservance across all the taxa over all the markers.