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Table 1 Summary statistics of screening of the small-insert partial genomic library of robusta coffee for putative SSR positive clones/sequences and SSRs.

From: Development of new genomic microsatellite markers from robusta coffee (Coffea canephoraPierre ex A. Froehner) showing broad cross-species transferability and utility in genetic studies

Summary of Screening/sequencing
Total Number of clones screened (X) 15,744
Number of clones selected and sequenced after screening 446 (2.83% of X)
Number of good quality sequences obtained 199 (1.27% of X)
Total number of SSR containing clones (Y) 76 (0.48% of X)
Number of sequences containing more than 1 SSR core 26 (34.21% of Y)
Number of sequences containing compound SSRs 15 (12.93% of Y)
Number of SSR+ sequences used for primer design/synthesis 58 (0.37% of X)
Number of working primer pairs 53 (0.34% of X)
Average size of the cloned/sequenced insert 773.5 bp
Haploid genome size of C. canephora [13] 809 Mb
Estimated genome screened (number of library clones x. average insert size) 12.2 Mb (1.5 % genome equivalent)
C. canephora genome sequenced (good quality sequences × average insert size) 0.15 Mb (0.01 % of robusta genome)
Summary of SSRs identified in the library
Number of non-targeted MNRs of minimum 12-mer length (a) 24 (0.15% of X)
Number of targeted DNRs having a minimum of 6 repeats (b) 46 (0.29% of X)
Number of non-targeted DNRs having a minimum of 6 repeats (c) 10 (0.06% of X)
Total number of DNRs (b+c) 56 (0.36% of X)
Number of targeted TNRs having a minimum of 5 repeats (d) 30 (0.19% of X)
Total number of DNRs and TNRs (b+c+d) 86 (0.55% of X)
Total Number of non-targeted HO-NRs having a minimum of 5 repeats (e) 6 (0.04% of X)
Total Number of DNRs, TNRs and HO-NRs (b+c+d+e) 92 (0.58% of X)
Total Number of SSRs (a+b+c+d+e) 116 (0.73% of X)