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Table 2 Phenotypic revertants were identified in the M2 generation among the progeny of EMS-treated cpr1 plants.

From: Phenotypic instability of Arabidopsis alleles affecting a disease Resistancegene cluster

Source

  

Number of M2 plants phenotype

   

cpr1

Intermediate

Wild-type

Treatment

Phenotype in M1

Family

   

Experiment #1

     

Mock3A

cpr1

c1

99

0

0

Mock

cpr1

c2

98

0

0

30 mM EMS

cpr1

c3

66

9

0

30 mM EMS3B

cpr1

c4

51

3

0

30 mM EMS3C

Chimeric

c5*

42

0

43

30 mM EMS

Chimeric

c6

21

0

18

Experiment #2

     

Treatment

     

Mock

cpr1

c7

85

0

0

Mock

cpr1

c8

96

0

0

30 mM EMS

cpr1

c9

50

3

0

30 mM EMS

cpr1

c10

28

0

0

30 mM EMS

Chimeric

c11

4

0

31

30 mM EMS

Chimeric

c12

31

0

74

  1. Plants with slightly curled leaves that were wider than cpr1 were called intermediate in phenotypic categorization. Chimeric M1 plants were those that developed chimeric sectors of wild type-looking leaves and stems. 3A, 3B, and 3C: Lines for which representative M2 plants are shown in Figure 3A to 3C.
  2. *: P < 0.05 by chi square test with expected ratio of 5:3 or 1:3 and degree of freedom = 1.