Figure 4

Subcellular localization of myosin VIII in abaxial leaf epidermis cells of N. benthamiana. Fluorescent chimeras were co-expressed by Agrobacterium infiltration. A. GFP-ATM1(IQ-tail). Scale bar 10 μm, 15 optic sections, 0.5 μm apart. B. GFP-ATM2 (IQ-tail) Scale bar 5 μm, 1 optic section. C. GFP-myosin VIIIA (IQ-tail). Scale bar 5 μm, 8 optic sections, 0.5 μm apart. D. GFP-ATM1(IQ-tail). E. RFP-ATM2(IQ-tail). F. Overlay of D and E. Scale bar 1 μm, 1 optic section. G. RFP-ATM1(IQ-tail). H. ERD2-GFP. I. Overlay of G and H. Scale bar 5 μm, 1 optic section. J. GFP-ATM1(IQ-tail). K. Aniline blue labeling of callose accumulated in pit fields. L. Overlay of J and K. Scale bar 5 μm, 1 optic section.