Figure 3
Stress conditions leading to GCN2-dependent eIF2α phosphorylation in Arabidopsis. A. Immunoblot analysis of eIF2α phosphorylation in wild type (WT) or gcn2 mutated seedlings. Seedlings were either left untreated (Ø), starved for purine with azA, exposed to low temperature (cold shock) or wounded on their leaves or roots. B. eIF2α phosphorylation in wild type untreated (Ø) seedlings, or seedlings exposed to NaCl, H2O2, low temperature (cold shock), or wounded on their leaves or roots (wound) C. eIF2α phosphorylation in wild type seedlings, untreated (Ø) or exposed to chlorsulfuron, salicylic acid, methyl jasmonate or 1-aminocyclopropane-1-carboxylic acid (ACC). In addition, for methyl jasmonate, eIF2α phosphorylation was already detected after 30 minutes of induction (Additional file 4).