Figure 4

Phylogenetic analysis of 34 Lx proteins. A parsimony tree was generated using bootstrap analysis with 1,000 replicates and branch swapping. Bootstrap values larger than 50 are denoted on each branch. The tree was rooted using Arabidopsis LOX1 (AtLOX1) and rice LOX1(OsLOX1). Soybean and Medicago Lxs in homologous regions represented as black and white squares fall into two major clades. For convenience, each Lx gene is named according to its species, chromosomal region and physical order instead of its GeneID. The Lxs in GmA diverged earlier from Lxs in GmB and MtB and later Lxs in GmB and MtB are separated, suggesting duplication before taxon divergence.