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Table 1 Mixture-amount treatment points and fresh- and dry-weight data for NE (nonembryogenic callus) and E (embryogenic callus).

From: The effects of nitrogen and potassium nutrition on the growth of nonembryogenic and embryogenic tissue of sweet orange (Citrus sinensis(L.) Osbeck)

Treatment Design Points Block Mixture Components Factor Fresh Wgt (Increase %) Dry Wgt (g) pHa
   NH4+ K+ NO3+ mM NE E NE E  
1 1 0.250 0.750 10.00 387 288 0.141 0.183 5.7
2 1 0.500 0.500 50.00 329 344 0.125 0.243 5.8
3 1 0.500 0.500 30.00 317 539 0.127 0.176 5.8
4 1 0.000 1.000 20.00 13 23 0.066 0.074 5.7
5 1 0.500 0.500 10.00 408 315 0.140 0.146 5.7
6 1 0.250 0.750 30.00 926 520 0.266 0.262 5.8
7 1 0.250 0.750 30.00 885 490 0.246 0.256 5.8
8 1 0.250 0.750 50.00 425 313 0.152 0.252 5.8
9 1 0.500 0.500 30.00 406 391 0.143 0.154 5.8
10 1 0.000 1.000 50.00 15 33 0.059 0.062 5.8
11 1 0.250 0.750 10.00 360 332 0.160 0.208 5.7
12 1 0.500 0.500 50.00 290 333 0.122 0.244 5.8
13 1 0.000 1.000 50.00 13 33 0.066 0.061 5.8
14 2 0.500 0.500 10.00 406 52 0.149 0.068 5.7
15 2 0.000 1.000 10.00 10 34 0.058 0.092 5.7
16 2 0.125 0.875 30.00 191 295 0.106 0.245 5.8
17 2 0.500 0.500 40.00 99 298 0.073 0.245 5.8
18 2 0.250 0.750 50.00 115 280 0.080 0.234 5.8
19 2 0.250 0.750 50.00 205 282 0.110 0.242 5.8
20 2 0.500 0.500 40.00 123 308 0.079 0.259 5.8
21 2 0.500 0.500 10.00 208 202 0.107 0.12 5.7
22 2 0.000 1.000 40.00 2 23 0.044 0.071 5.8
23 2 0.000 1.000 10.00 12 24 0.056 0.084 5.7
24 2 0.000 1.000 10.00 9 33 0.050 0.096 5.7
25 2 0.250 0.750 20.00 403 493 0.156 0.241 5.8
26 2 0.000 1.000 40.00 15 71 0.0702 0.095 5.8
  1. Experiment is a two-component mixture of NH4+ and K+ and one quantitative factor NO3- amount. The mixture components are listed as proportions with their actual amounts matched to the amount of NO3-. For example, treatment point #1 included 2.5 mM NH4+, 7.5 mM K+, and 10 mM NO3-. The data represent the mean of six duplicate plates per treatment point. Points #17 and #20 are MS medium.
  2. a – Calculated from the chemical equilibrium modeling software MINEQL+ Ver. 4.5 (27), temperature corrected and assumed open to the atmosphere with a PCO2 at sea level of 10-3.50 atm.