The effects of nitrogen and potassium nutrition on the growth of nonembryogenic and embryogenic tissue of sweet orange (Citrus sinensis(L.) Osbeck)

Table 1 Mixture-amount treatment points and fresh- and dry-weight data for NE (nonembryogenic callus) and E (embryogenic callus).

Treatment Design Points	Block	Mixture Components		Factor	Fresh Wgt (Increase %)		Dry Wgt (g)		pH^a
		NH₄⁺	K⁺	NO₃⁺ mM	NE	E	NE	E
1	1	0.250	0.750	10.00	387	288	0.141	0.183	5.7
2	1	0.500	0.500	50.00	329	344	0.125	0.243	5.8
3	1	0.500	0.500	30.00	317	539	0.127	0.176	5.8
4	1	0.000	1.000	20.00	13	23	0.066	0.074	5.7
5	1	0.500	0.500	10.00	408	315	0.140	0.146	5.7
6	1	0.250	0.750	30.00	926	520	0.266	0.262	5.8
7	1	0.250	0.750	30.00	885	490	0.246	0.256	5.8
8	1	0.250	0.750	50.00	425	313	0.152	0.252	5.8
9	1	0.500	0.500	30.00	406	391	0.143	0.154	5.8
10	1	0.000	1.000	50.00	15	33	0.059	0.062	5.8
11	1	0.250	0.750	10.00	360	332	0.160	0.208	5.7
12	1	0.500	0.500	50.00	290	333	0.122	0.244	5.8
13	1	0.000	1.000	50.00	13	33	0.066	0.061	5.8
14	2	0.500	0.500	10.00	406	52	0.149	0.068	5.7
15	2	0.000	1.000	10.00	10	34	0.058	0.092	5.7
16	2	0.125	0.875	30.00	191	295	0.106	0.245	5.8
17	2	0.500	0.500	40.00	99	298	0.073	0.245	5.8
18	2	0.250	0.750	50.00	115	280	0.080	0.234	5.8
19	2	0.250	0.750	50.00	205	282	0.110	0.242	5.8
20	2	0.500	0.500	40.00	123	308	0.079	0.259	5.8
21	2	0.500	0.500	10.00	208	202	0.107	0.12	5.7
22	2	0.000	1.000	40.00	2	23	0.044	0.071	5.8
23	2	0.000	1.000	10.00	12	24	0.056	0.084	5.7
24	2	0.000	1.000	10.00	9	33	0.050	0.096	5.7
25	2	0.250	0.750	20.00	403	493	0.156	0.241	5.8
26	2	0.000	1.000	40.00	15	71	0.0702	0.095	5.8

Experiment is a two-component mixture of NH4+ and K+ and one quantitative factor NO3- amount. The mixture components are listed as proportions with their actual amounts matched to the amount of NO3-. For example, treatment point #1 included 2.5 mM NH4+, 7.5 mM K+, and 10 mM NO3-. The data represent the mean of six duplicate plates per treatment point. Points #17 and #20 are MS medium.
^a – Calculated from the chemical equilibrium modeling software MINEQL+ Ver. 4.5 (27), temperature corrected and assumed open to the atmosphere with a P_CO2 at sea level of 10^-3.50 atm.

ISSN: 1471-2229