Figure 8

Schematic representation of Tgmt* Gene-1 and cloned cDNAs. The amplification products shown in Figures 5 and 6 were cloned and their sequences aligned to determine the exon-intron boundaries. Gene-1 larger exons are shown boxed and numbered on a solid line that represent the introns and the 5'- and 3'-ends of Gene-1. The smallest exons (-9, -12, -16, -18, -20, -23, -24) are represented by two parallel lines. Left (L) and right (R) borders are reduced in size to fit the size scale. L is 6,122 bp and R, 1,465 bp. Solid arrow heads represent the CACTA terminal inverted repeats. The two unfilled arrows above the diagram of the transposon represent ORF1 with the transposase tnp2 domain (tnp2) and ORF2 with the TNP1 transposase domain. Below the Tgmt* diagram are the cDNAs amplified with the different sets of primers shown in Figures 6 and 7. The numbered primers are sketched with small arrows underneath Tgmt* or near the cDNAs to simplify the figure. The splice site of the mosaic cDNAs amplified with primers 1 and 7 is indicated with an asterisk (*). The 1/9 portion of Exon-1 represents the 346 bp of Exon-1 that are spliced to Intron-10 right border. Cl = clone number.