Figure 7

Tgmt* Gene-1 expression in mutable 33745 ( t^m) line. DNA fragments amplified in RT-PCR reactions using four different sets of primers and RNAs from the mutable 33745 (t^m) line were visualized in EtBr stained agarose gels. The primer numbers are indicated at the bottom of the figure for each set of RT-PCR reactions. A) 1 and 6; 1 and 7; 5 and 7. B) 3 and 6. See Methods for sequences of DNA primers. The negative controls (-) were reactions in which the cDNA synthesis step was allowed in the absence of superscript.