Figure 1

Amplification of a large DNA insertion in Intron-1 of F3'H alleles. DNA fragments amplified from genomic DNAs isolated from 5 soybean lines with different T locus alleles were visualized in this EtBr stained gel: Williams 43 (T), XB22A (T*), 33745 (t^m), 37643 (t*) and 37609 (t*). The ~23 kb fragment contains a large insertion in Intron-1 of the t* allele. The 2.6 kb fragment is the Intron-1 DNA portion between the primers chosen for the PCR reactions (Z37F and IN663; see Additional file 5: Alignment of cDNA sequences of clones 43–53 to Tgmt* genomic sequence, and Methods). M is a 1 kb lambda DNA ladder. M2 is a HindIII lambda DNA marker.