Table 3 Stress-induced transcriptional changes of genes involved in starch and lipid mobilization pathways.a
Reaction | Enzyme | GenBank accession number of EST showing similarity to the enzyme | Maximal ratio of transcript abundance (multistress:standard) | Time point of maximal transcriptional differences |
|---|---|---|---|---|
Starch degradation | ||||
1 | Alpha-amylase (EC 3.2.1.1) | FG345587 | 3.1** | T1 |
1 | Alpha-amylase (EC 3.2.1.1) | FG344655 | 1.9* | T1 |
2 | Beta-amylase (EC 3.2.1.2) | FG345593 | 3.0* | T1 |
Glycolysis | ||||
4 | Phosphoglucomutase (EC 5.4.2.2), cytosolic | BQ586122 | 2.7** | T50 |
8 | Fructose-bisphosphate aldolase (EC 4.1.2.1), cytosolic | FG343104 | 1.5 | Tmax |
8 | Fructose-bisphosphate aldolase (EC 4.1.2.1), plastidic | FG345732 | 2.0* | T50 |
9 | Triosephosphate isomerase (TPI) (EC 5.3.1.1), cytosolic | FG344285 | 2.2 | T1 |
12 | Phosphoglycerate mutase (PGAM) (EC 5.4.2.1), cytosolic | BQ583729 | 2.3* | T1 |
14 | Pyruvate kinase (PK) (EC 2.7.1.40), cytosolic | FG344735 | 1.5 | T1 |
Pyruvate decarboxylation and fermentation | ||||
15 | Pyruvate dehydrogenase E1 alpha subunit (EC 1.2.4.1) | FG345415 | 1.8* | T1 |
16 | Alcohol dehydrogenase (EC 1.1.1.1) | FG343474 | 1.5 | T1 |
Fatty acid beta-oxydation | ||||
17 | Acyl-CoA synthetase (EC 6.2.1.3) | FG344059 | 1.9* | T50 |
19a | Enoyl-CoA hydratase (EC 4.2.1.17) | FG345194 | 0.4** | Tmax |
20 | Thiolase (EC 2.3.1.9) | FG344716 | 0.5*** | Tmax |
Glyoxylate cycle | ||||
21 | ATP citrate synthase (EC 2.3.3.8) | FG345121 | 0.7* | Tmax |
23 | Isocitrate lyase, glyoxysomal (EC 4.1.3.1) | FG345539 | 0.5** | Tmax |
Gluconeogenesis | ||||
26 | Phosphoenolpyruvate carboxykinase (EC 4.1.1.49) | FG343814 | 0.5** | Tmax |