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Table 4 secondary structure formation analysis at 5' splice sites of AFEs

From: Systematic analysis of alternative first exons in plant genomes

  Constitutive (± SD) * AFE Type I AFE Type II
   Total Major** Minor** Total Major** Minor**
Rice -19.22 ± 5.59 -23.61 ± 8.62 -24.28 ± 8.37 -23.00 ± 8.79 -22.45 ± 7.8 -24.7 ± 8.51 -20.37 ± 6.46
Comparison with constitutive sites ***   3.2796e-071 1.8749e-061 9.6957e-035 9.6069e-082 1.7511e-160 3.0208e-012
Arabidopsis -17.80 ± 4.33 -15.09 ± 5.10 -14.59 ± 5.38 -15.60 ± 4.62 -16.52 ± 4.98 -16.47 ± 4.89 -16.46 ± 5.29
Comparison with constitutive sites ***   1.6711e-028 4.5892e-022 1.3987e-011 4.7938e-015 1.9863e-009 2.9444e-009
  1. * Secondary structure formation was measured as Minimal Folding Energy (MFE) by MRNAFOLD. Lower scores indicate a higher likelihood of an input sequence to form a secondary structure;
  2. ** Major and minor types of alternative first exons within each gene cluster were determined as described in the Methods section.
  3. *** P-values were determined using t-tests.