Figure 6

Analysis of cell death in Physcomitrella. Evans blue staining of protonemal tissues after treatments with LB (A), CF_(SCC3193) (B), CF_(SCC1) (C), PDB (D) and B. cinerea spores (E). A closer view of CF_(SCC3193)- (F), CF_(SCC1)- (G) and B. cinerea inoculated (H) protonemal cells is shown. Arrows indicate cytoplasmic shrinkage. Leaves treated with CF_(SCC1) (I) and B. cinerea spores (J) were also stained with Evans blue. Pictures of representative tissues were taken 2 days after treatment.