Figure 4

Correlation of growth phase and poly P staining of the cell wall. A, Asynchronously growing wild type mt⁺ 137c cells were stained for cell wall poly P. The cells were assigned to four different states of development during cytokinesis and categorized visually according to fluorescence intensity of the cell wall ("strong": Cy2 signal brighter than cholorophyll signal; "intermediate": Cy2 similar or slightly fainter than chlorophyll; "faint": Cy2 visible, but much fainter than chlorophyll; "none": no Cy2 signal visible). B, The numbers of total cells and mother cells at various time points indicate synchronous cell division of wild type mt⁺ 137c cells. C, Cells from this synchronously growing culture were stained at various time points during mitosis and analysed by confocal microscopy (green: poly P staining, red: chlorophyll).