Characterization of AtCNGC10 associated with vesicles and cell plate. (A) Immunogold labeling of cytoplasmic vesicle (V, black arrow) and vesicles merging with plasma membrane, pm (white triangles). Black arrowhead indicates 15 nm immunogold in pm. (B) Three immunogold particles from anti-AtCNGC10 antiserum labeling on edge of vesicle (V); (C) Cell plate (cp) formation with anti-AtCNGC10 antiserum labeling indicated with black arrowheads. Endoplasmic reticulum (er). (D) Multiple vesicles present in cytoplasm labeled with anti-AtCNGC10 antiserum; (E) Anti-AtCNGC10 antibody labeling near cortical microtubule (MT) attachments. cw, cell wall. Size bars are in nanometers (nm).