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Figure 1 | BMC Plant Biology

Figure 1

From: The cyclic nucleotide gated cation channel AtCNGC10 traffics from the ER via Golgi vesicles to the plasma membrane of Arabidopsis root and leaf cells

Figure 1

Structure and expression of CNGCs in Arabidopsis. (A) Two dimensional model of a generic CNGC as positioned in the membrane relative to the inward and outward orientations. Locations of the epitopes for the AtCNGC10- and AtCNGC5-specific antisera are shown. Six transmembrane domains (S1-S6), the pore (PR), cyclic nucleotide-binding (CNBD) and calmodulin-binding domains (CaMBD) are indicated. (B) Quantitation of relative mRNA levels for the Atcngc10 and Atcngc5 genes using quantitative RT-PCR. Cngc mRNA levels were normalized to actin mRNA levels. (C) Immunoprecipitation of AtCNGC10 and AtCNGC5 from root (Rt) and leaf (Lf) total cellular proteins vs. a negative control (Cn, E. coli total protein). Molecular sizes of protein bands are indicated in kilodaltons (kDa). The black arrowhead denotes the band corresponding to each CNGC. The asterisk (*) refers to a non-specific band detected in all samples.

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BMC Plant Biology

ISSN: 1471-2229

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