Constitutive expression of mitochondrially targeted eqFP611. Protoplasts derived from stably transformed N. tabacum plants constitutively expressing eqFP611 targeted to mitochondria (pIVD145-eqFP611-pBI121). (A) Image taken through MitoTracker filter set. Scale bar: 10 μm. (B) Plasmid used for transformation. The IFP expression cassette is identical with that in Figure 2, but has been inserted into pBI121. Kanr: kanamycin resistance cassette (NOS promoter, neomycin phosphotransferase II, NOS terminator). RB: right border, LB: left border. Vector backbone: pBI121.